Analysis of Polyphyllin II alters microRNA expression profile in lung cancer A549 cells

  • Weidong Peng Sinopharm Dongfeng General Hospital, Hubei University of Medicine, Shiyan 442008, China
  • Huimin Huang Sinopharm Dongfeng General Hospital, Hubei University of Medicine, Shiyan 442008, China; Department of Pharmacy, Xi’an Jiaotong University, Xi’an 710003, China
  • Jingjian Liu Sinopharm Dongfeng General Hospital, Hubei University of Medicine, Shiyan 442008, China
  • Binqiang Zhang Sinopharm Dongfeng General Hospital, Hubei University of Medicine, Shiyan 442008, China
  • Lun Wu Sinopharm Dongfeng General Hospital, Hubei University of Medicine, Shiyan 442008, China
  • Zhongzhi Liu Sinopharm Dongfeng General Hospital, Hubei University of Medicine, Shiyan 442008, China
  • Fengying Ran Sinopharm Dongfeng General Hospital, Hubei University of Medicine, Shiyan 442008, China; Department of Pharmacy, Xi’an Jiaotong University, Xi’an 710003, China
DOI: https://doi.org/10.62617/mcb.v21i1.212
Keywords: polyphyllin II; miRNA; lung cancer; A549 cells
Ariticle ID: 212

Abstract

Non-small cell lung cancer (NSCLC) is a malignant tumor that threatens the whole world, previous studies found that Polyphyllin II (PPII) can suppress NSCLC cells growth, exhibits significant antitumor activity. MicroRNAs (miRNAs) can regulate the expression of other genes and play a crucial role in the prevention and development of cancer. However, the miRNA portrait of ginsenoside PPII-treated NSCLC A549 cells has not yet been studied. In this work, miRNA-seq analysis was used to determine the changes in miRNA expression profile of NSCLC A549 cells PPII-treaded. In addition, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis were performed on differentially expressed miRNA target genes. Then, predicted the target genes of miRNA and performed functional enrichment analysis on them. We identified 58 up-regulated and 24 down-regulated miRNAs displaying changes their expression in PPII treated A549 cells were greater than 2-fold. In addition, the expression levels of miRNAs were validated by real-time PCR. Therefore, this work has a certain promoting effect on the study of the anti-cancer mechanism of PPII in lung cells.

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Published
2024-11-04
How to Cite
Peng, W., Huang, H., Liu, J., Zhang, B., Wu, L., Liu, Z., & Ran, F. (2024). Analysis of Polyphyllin II alters microRNA expression profile in lung cancer A549 cells. Molecular & Cellular Biomechanics, 21(1), 212. https://doi.org/10.62617/mcb.v21i1.212
Issue
Vol. 21 No. 1 (2024)
Section
Article

Copyright (c) 2024 Weidong Peng, Huimin Huang, Jingjian Liu, Binqiang Zhang, Lun Wu, Zhongzhi Liu, Fengying Ran

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