Unraveling molecular mechanisms in growth plate development advancing pediatric orthopedic interventions
Abstract
Dynamic Growth Plate based on Molecular Profiling (DGMP) represents a pioneering approach to understanding the intricate molecular processes governing growth plate development, essential for advancing pediatric orthopedic treatments. Growth plates, located at the ends of long bones, are pivotal for bone elongation during childhood and adolescence, but disruptions in their molecular and cellular regulation can result in developmental abnormalities and orthopedic deformities. DGMP integrates genomic, transcriptomic, proteomic, and epigenomic analyses to uncover cell-specific expression patterns and regulatory elements critical to growth plate formation. By leveraging high-throughput sequencing, single-cell RNA (Ribonucleic Acid) sequencing, and spatial transcriptomics, DGMP facilitates the identification of diagnostic biomarkers and enables the development of targeted pharmacological therapies tailored to children with defective growth plates. Furthermore, in silico models simulating cellular differentiation and pathway interactions provide predictive insights into the long-term effects of interventions on bone development. This innovative framework not only enhances early and accurate detection of growth-related disorders but also supports the design of personalized treatments, ultimately improving clinical outcomes for affected children. As a precision medicine tool, DGMP has the potential to transform pediatric orthopedics by resolving challenges related to molecular data integration, spatiotemporal dynamics, and therapeutic application, thereby advancing the understanding and treatment of growth plate disorders.
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